Summary

We, the iGEM Team Freiburg 2014, managed to combine the capability of viral vectors for stable gene transfer with the spatial resolution of optogenetics for gene delivery in a spacio-temporal manner. We generated patterns by illuminating discret areas in a cell culture with light of a special wave lenght and infected target cells with the viral vector containing the gene of interest, afterwards. Stable integrated our gene cells were expressing a fluorescence protein visible via microscopy. On the same principle cells were infected with a viral vector containing a gene for SEAP. Resulting in colour changing of the medium we generated QR codes on multiple well plates.