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Team:UT-Tokyo/CTCD/Content
From 2014.igem.org
The word, "counter", will remind you the machine with which you can count the number of the objects, such as people and cars. Some people familiar with electronic circuits may remember the logic circuit. In any case, both are the memory device of the order of inputs and are important for our lives.
Cellular counters also memorize the number of events. In nature, there are tellomere length regulation, cell aggregation and so on.
Ari et al constructed the cellular counter with synthetic biological approach, termed the riboregulated transcriptional cascade (RTC) counter[1].This counter moves the states after arabinose induction (Fig. 1). The system is regulated by translational regulation with riboregulators. The Biobrick part of this cellular counter has already existed, which was constructed by Tokyo-Nokogen 2009 and is named BBa_K225002 [2].
In order to expand the function of this counter, we added reset system. The reset sysytem realizes the movement from any state to the initial state after the particular input and is expected to movement from down stream states to upstream states by respective inputs, which we regard as automaton. Our counter utilizes the regulation system of sigma factor and anti-sigma factor as the key of its resetting mechanism. In addition to reset function, the number of states can be increased more easily, because what we have to think is only the combination of sigma and anti-sigma factor, that is, we do not have to find new polymerase. Moreover, because there is orthonal relation between sigma and anti-sigma factor, we can choose combinations which help us avoid crosstalk problem.
[1]Ari E. Friedland, et al., Science 324, 1199 (2009).
[2]https://2009.igem.org/Team:Tokyo-Nokogen/Parts
Fig. 1 The concept of RTC counter. After first, second and third induction of arabinose, the state of cells moves from 0 to 1, 1 to 2 and 2 to 3, respectively.
iGEM UT-Tokyo
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iGEM UT-Tokyo
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iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
iGEM UT-Tokyo
MASAKI ONO
- Name
- Masaki Ono
- Belong to
- The University of Tokyo, Literature, Sophmore
- Job
- Modeling
I want to be a doctor.
The word
