"
Team:Paris Bettencourt/Project/TMAU
From 2014.igem.org
| BACKGROUND
Trimethylaminuria (TMAU) commonly called Fish Odor Syndrom, is a rare genetic disease; trimethylamine (TMA) is naturally produces in the gut by the intestinal flora but it supposed to be degraded in the liver by the FMO3 enzyme coded by a gene with the same name. The suffering patients have a mutated FMO3 gene, and TMA is excreted in sweat, saliva and urine, causing a strong fish odor. |
AIMS
tmm (trimethylamine monooxygenase) gene is a bacterial gene found in the bacteria Ruegeria pomeroyi and is similar to the human FMO3 gene. The aim of this project is to clone tmm into Corynebacterium striatum, a bacteria of the skin, and to incorporate the new strain in a probiotic cosmetic in order to cure fish odor. |
RESULTS
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc convallis fringilla nunc, nec tristique neque. Praesent ut ante ultrices, dignissim nunc eget, interdum velit. Donec lobortis iaculis lorem, eget ornare quam finibus et. Vestibulum vehicula fringilla mauris, vitae feugiat ligula pharetra a. Duis fermentum neque vel dapibus accumsan. Quisque fringilla. |
BIOBRICKS
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Mauris vel neque odio. Suspendisse eget dui quis quam semper placerat at nec orci. Cras malesuada sem nec aliquam imperdiet. Cras vitae nisl eu augue iaculis aliquam et eget turpis. Fusce pulvinar ipsum eu neque auctor, at luctus nibh pulvinar. Vivamus in. |
| Introduction | Aims | ? | Results | Motivations |
Introduction
Trimethylamine (TMA) is produced in the intestine by Desulfovibrio desulfuricans thanks to the fermentation of choline. In healthy patients, the FMO3 gene allows the degradation of TMA in the liver into a non-volatile compound, TMA oxide. But a mutation in the FMO3 sequence is most of the time the cause of TMAU: TMA is not degraded and is then excreted in sweat, saliva and urine leading to a strong fish odor. The patients are otherwise healthy but the disease affect their social relationships and can lead to depression. There is no cure for this metabolic disorder but some treatments, often based on avoiding some sorts of food, tend to lower the symptoms.
Aims
The strain Ruegeria pomeroyi, a genus of the Rhodobacteraceae, produces an enzyme called trimethylamine monooxygenase thanks to the tmm gene. As FMO3, this enzyme degrades trimethylamine into trimethylamine-N-oxide but is adapted to a bacterial expression. The project aims at cloning tmm into E.coli and then into Corynebacterium striatum, one of the most common bacteria of the skin. The new strain would be integrated to the skin microbiome and would suppress the fish odor.
Results
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed sit amet laoreet metus, ac viverra dolor. Sed et orci imperdiet sem vulputate ultricies. Aliquam erat volutpat. Cras semper ex non odio aliquet, eget feugiat eros tempor. Integer hendrerit odio et bibendum maximus. Duis scelerisque lacus in odio faucibus fringilla. Nulla eleifend aliquet molestie. Morbi aliquam rhoncus efficitur. Proin consectetur augue aliquam risus convallis egestas. Nunc viverra felis non nibh consequat, nec faucibus ipsum rutrum. Proin placerat faucibus libero vitae dapibus.
Motivation
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam eu justo a dolor efficitur laoreet ut at lorem. Fusce dapibus lobortis nisi vehicula porttitor. In volutpat mauris et aliquam pellentesque. Vestibulum fringilla lacus metus, ac ullamcorper lectus sagittis sed. Suspendisse congue magna sed risus molestie aliquam. Sed placerat sagittis volutpat. Phasellus id erat neque. Quisque bibendum iaculis ante et feugiat. In hac habitasse platea dictumst. Fusce placerat lorem vel felis tincidunt, in elementum odio condimentum. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Donec tincidunt bibendum lacus non viverra. Nulla mattis, ante vitae faucibus auctor, mi purus consequat dolor, non malesuada nulla lorem ac odio.
