Team:TU Delft-Leiden/Project/Life science/EET/cloning

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Revision as of 21:32, 30 September 2014 by Joanctd.igem (Talk | contribs)

Extracellular Electron Transport Module - Cloning

Final constructs

The final BioBricks constructed for this module were:
  • mtrCAB

  • mtrCAB - His tag

  • ccmAH

  • mtrCAB

    Important note
    It is important to notice that the actual assembly used the 5 fragments PCRed from the original Ajo-Franklin plasmid containing the mtrCAB genes. In the figure it might seem that this happened in two steps, but that is due to the Software used to check the assembly "in silico" (SnapGene), which was limited to an assembly of four fragments.

    mtrCAB - His tag

    ccmAH

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