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Team:TU Delft-Leiden/WetLab/landmine/cloning/yqjF
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Revision as of 13:20, 30 September 2014 by Joanctd.igem (Talk | contribs)
Landmine Module - Cloning - yqjF promoter
yqjF promoter
Two final constructs are based on the yqjF promoter alone: BBa_K1316003 and BBa_K1316005. For both of them the reporter gene mKate2 was placed behind the yqjF promoter. As BBa_K1316005 was built on bases of BBa_K1316003, a double transcriptional terminator was placed after the mKate2 gene. Otherwise, the RNA polymerase would continue transcribing and some unfinised transcripts of the N-genes could interfere with the normal expression of these genes. Therefore, mKate2 gene was first introduced in front of a Double terminator ( BBa_K823017 )
