Team:Goettingen/proLP

Overview

PCR Methods

• Fusion PCR
• Gradient PCR

DNA Assessment

• Gel Extraction
• DNA Gel Electrophoresis
• Pretreatment of DNA for Sequencing
• Fluorescence Assay

Plasmid Construction

• BP recombination reaction
• LR recombination reaction

Plasmid Transformation

• E.coli competent cells
• E.coil transformation
• Plasmid isolation
• Yeast transformation

Colony Scanning

• E.coli cracking
• Yeast colony PCR
• Auto-activity Test
• Yeast-Two-Hybrid Screening
• Y2H blue/white selection