Our laboratory is certified for Biosafety Level 1 (BS-1) work, and we have access to a Biosafety Level 2 lab for BSL2 work. Our work fell within the BSL-1 domain, as indicated by the Center of Disease Control guidelines. All biological waste was stored in autoclave bags and was autoclaved prior to disposal. Sharps and broken glassware were disposed of according to institutional guidelines. Hazardous liquid waste was clearly labeled, and stored in secondary containment for disposal by the institution. Thus, although there is potential for harm to researchers, it is minimized through following procedures approved and used by many laboratories at Yale. It is also minimized by training and common sense. E. coli strains used were common laboratory strains and not pathogenic.
All students working in the laboratory were required to complete the following set of training tutorials, including passing a test at the end. http://ehs.yale.edu/training/biosafetypartI http://ehs.yale.edu/training/biosafetypartII http://learn.caim.yale.edu/chemsafe/login.html http://ehs.yale.edu/training/hazardous-chemical-waste-management
All materials were used in accordance with local, national, and Yale Biosafety requirements. Standard lab practices were followed, including secondary containment of chemicals, proper storage of volatiles and flammables, and separation of acids and bases. Nitrile gloves were worn at all times within the lab. A pipet was kept exclusively for ethidium bromide use. Fume hoods were used when handling volatile compounds, concentrated acids and bases, and other reagents. Inhalation and skin contact was avoided. Chemical agents were properly disposed of in designated biohazard waste bins. When UV light was used to visualize gels or GFP, special care was taken to avoid skin or eye exposure. Absolutely no food was allowed in the lab.
Our project was overseen by the Yale Biological Safety Committee and the Office of Environmental Health and Safety (OEHS). Our project has been approved as consistent with Yale's safety regulations. No changes to our project were required since proper protocols were followed. Training was completed as described above.