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Team:Bielefeld-CeBiTec/HumanPractice/Synenergene/RiskAssessment
From 2014.igem.org
SYNENERGENE
Risk assessment
Of great interest for an application are the risks. The advantage of our system is that the cells grow in a closed system. This makes it easier to solve biosafety issues. Next to our main project we aim to develop a selection system without antibiotics. The advantage of this is that the resulting product has no traces of antibiotics which is a benefit for many industrial productions. If the strain loses his plasmid it will die because of the missing alanin racemase which produces D-alanin. D-alanin is a key substance for the cell wall of bacteria which they cannot produce in our strain. In addition we implemented a biosafety system which is dependend on rhamnose (figure 11). Without rhamnose the RNase Ba (Barnase [Bacillus amyloliquefaciens]) will be produced in addition to the end of the expression of the alanin racemase. This leads to lysis of the cell wall and degradation of the RNA (figure 12). Our strain is not dangerous in biosecurity terms because it has no pathogenicity. In addition it has no relevant modifications which can be abused.
 fig. 11: With L-rhamnose |
 fig. 12: Without L-rhamnose |
