Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jun

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June

  • Initial cultivation experiments with neutral red
    • This week we wanted to test the properties of neutral red during a cultivation on a small scale
      • Cultivation of the E. coli strain with porines (attempt 1) and wildtyp as control (attempt 2)in LB-medium with 100 µM neutral red
      • pH was set up at 8.75
      • Results
        • Attempt 1 turned red (pH = 7.495)
        • Attempt 2 remained yellow
  • Reduction and Oxidation of neutral red
    • This week we checked the properties of neutral red under oxidizing and reducing conditions by adding chemical agents
      • Reducing agent: sodium dithionite
        • Red solution got reduced and turned colorless
        • Got oxidised by air again afterwards
      • Oxidizing agent: hydrogen peroxide
        • Red solution stayed red
        • Yellow solution stayed yellow
      • Conclusion: the color of the solution depends on the pH