Calender

Click on a date to see our research activities on that day.

Glossary

His

alphabet

GFP

Green Fluorescence Protein

RFP

Red Fluorescent Protein
This is BBa_E1010.

5'-intron

5'-intron M

3'-intron

Lac

LacI regulated promoter.
This is BBa_R0010.

Amp

Ampicillin resistance gene

Cm

Chloramphenicol resistance gene.
This is pSB1C3.

RBS

Ribosome Binding Sites.
This is BBa_B0034.

DT

Double Terminator consisting of BBa_B0010 and BBa_B0012.
This is BBa_B0015.

EcoRI

Restriction site that is cut by EcoRI or restriction enzyme

XbaI

Restriction site that is cut by XbaI or restriction enzyme

SpeI

Restriction site that is cut by SpeI or restriction enzyme

PstI

Restriction site that is cut by PstI or restriction enzyme

TTHA0715

Cold shock protein with amino acid sequence cut by thrombin

TTHA0715 guanidine deposition

SmtA

circular RNA

Prologue -before August-

Parts amplification with PCR

1. Lac
2. RBS
3. RFP
4. DT
5. Cm (pSB1C3)
6. pSB1A3
7. pSB1K3

T4 phage Multiplication with E.coli

DNA Extraction from T4 phage

August

August 11th

Restriction Enzyme Digestion

Ligation

1. EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
2. EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
3. EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
4. EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI

Transformation

1. -His-RFP(with stop codon)-DT-Cm-
2. -5'intron M-DT-Cm-
3. -Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm-
4. -His-RFP(without stop codon)-Amp-

Densitometry