Team:Oxford/biosensor construction
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Construction
Introduction: how we constructed our biosensor
In order to be able to use our model and to determine whether DcmR acts as a repressor or activator in the presence of DCM we designed and constructed the following two plasmid system. We primarily used Gibson assembly methods and source most of the necessary DNA from gblocks(synthesised oligonucleotides) we had designed based in the sequenced genome of Methylobacterium DM4. This system will also form the DCM biosensor and will be integrated with an elctronic circuit to complement this genetic one:![](https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png)
![](https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png)
![](https://static.igem.org/mediawiki/2014/0/08/Oxford_Fran_flowchart2.png)
![](https://static.igem.org/mediawiki/2014/8/84/Oxford_Fran_flowchart1.png)
Building pOXON-1
Building pOXON-2 and pOXON-2-dcmR
Adding in mCherry
Building pSRK Gm construct
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