"
Team:Penn/Notebook
From 2014.igem.org
Notebook
- Week 1
- Molecular Biology Training Workshop
- Practiced the basics of molecular cloning
- Week 2
- Idea Brainstorming and Generation
- Compiled a preliminary list of potential ideas
- Week 3
- Settled on two main ideas: 1. quorum sensing with antibiotics 2. Heavy metal removal with Magnetotactic bacteria
- Learned how to use Geneious to design cloning process
- Practiced extracting biobricks and transforming NEB Turbo cells with plasmids
- Visited Biomeme to get the portable qPCR machine
Human Practices
- Week 4
- Decided on the project idea: Heavy metal removal with Magnetotactic bacteria
- Identified primers and promoters in AMB-1 strain
- Identified AMB-1 transformation vector
- Extracted smtA biobrick gene and made glycerol stock
- Week 5
- Developed three goals to accomplish for the project
- Determined the constructs to clone into AMB-1
- Designed two fast-fail experiments
- Created a workflow for the construction of plasmid
- Planned outreach events at high school summer programs
Human Practices
- Week 6
- Ordered chemicals for AMB-1 growth medium
- Completed primer design
- Obtained AMB-1 strain from Dr.Goulian
- Week 7
- Determined the OD600 plate reading conversion formula experimentally
- Attempted to do E.Coli and AMB-1 cadmium tolerance test
- Designed all construct on Geneious
- Met with Dr.Rizk to discuss presenting to incoming bioengineering freshmen
Human Practices
- Week 8
- Ordered DNAs from Genscript and IDT
- Week 9
- Redid E.Coli Cadmium Tolerance Test in M9 media
- Learned to do cell count for AMB-1
- Attempted to make AMB-1 chemically competent
- Presented to high school students at Penn M&T program
- Planned a synthetic biology preceptorial
- Contacted Schuylkill Action Network
Human Practices
- Week 10
- Completed AMB-1 growth curve under different media
- Determined cell count and OD600 conversion formula for AMB-1 experimentally
- Completed E.Coli Cadmium Tolerance Test in LB media
- Volunteered at SEA Science Carnival
Human Practices
