Team:Oxford/biosensor construction
From 2014.igem.org
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Construction
Introduction: how we constructed our biosensor
In order to be able to use our model and to determine whether DcmR acts as a repressor or activator in the presence of DCM we designed the following two plasmid systems:![](https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png)
![](https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png)
![](https://static.igem.org/mediawiki/2014/0/08/Oxford_Fran_flowchart2.png)
![](https://static.igem.org/mediawiki/2014/8/84/Oxford_Fran_flowchart1.png)
Building pOXON-1
Building pOXON-2 and pOXON-2-dcmR
Adding in mCherry
Building pSRK Gm construct
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