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Team:Evry/Interlab Study/09-18-2014
From 2014.igem.org
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.
PSB1C3+E0240+J23102:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.
PSB1C3+E0240+J23104:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.
PSB1C3+E0240+J23105:
- glycerol stock preparation from LB culture from the 17th September.
- Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.
PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture from the 17th September.
PSB1C3+E0240+J23117:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.
Sep 18
