In order to be able to use our model and to determine whether DcmR acts as a repressor or activator in the presence of DCM we designed the following two plasmid systems:
The two plasmids are partitioned during cell division by different systems, thus an equal proportion of each plasmid is maintained in each new daughter cell.
Different antibiotic resistances will allow us to select for cells that have taken up both plasmids by application of both antibiotics.
The replication origins compatible with E.coli and pseudomonas strains.
We have used two plasmids so that we can test each part in isolation before transforming them both into the same cell.
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Oxford iGEM 2014
Oxford iGEM 2014
