Team:Evry/Interlab Study/09-11-2014

Constructions with other promoters

PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118:

• Transformation plate from the 10th observation. There were 50 colonies for each constructions.
• PCR colony of 8 colonies for each construction following protocol table 1 and 2. https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
  

  

  1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction.
• PCR purification of sample 1 and 4 with the were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
• Preparation of samples to sequencing. N° XX
• Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.

PSB1C3+E0240+J231xx:
Sep 11