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From 2014.igem.org

Contents 1 Lab Work 1.1 D-lemon scent 1.1.1 Transformation with the ligation (futur pPS2/4) 1.1.2 liquide culture of some PS clone (topo) 1.1.3 Transformation of LS GS CAS (topo) to do stock 1.1.4 PCR pSCBC3 cloning 1.2 B-B - Construction of the fusion protein (color) 1.2.1 PCR 1.2.2 transformation for stock of the w6 clone

Lab Work

by Mélanie

D-lemon scent

Transformation with the ligation (futur pPS2/4)

liquide culture of some PS clone (topo)

Transformation of LS GS CAS (topo) to do stock

PCR pSCBC3 cloning

PCR of GS : primer with ecoRI and PSTI restriction site

B-B - Construction of the fusion protein (color)

PCR

We have decided to separate the two protein So I do a PCR to have the yellow and the blue protein and i cloned them in a topo plasmid.

transformation for stock of the w6 clone