"

From 2014.igem.org

Contents 1 Lab Work 1.1 D-Lemon Scent 1.1.1 Plasmid extraction 1.1.2 digestion 1.1.3 Purification 1.1.4 culture 2 Modeling

Lab Work

by Mélanie

D-Lemon Scent

Plasmid extraction

From the liquide culture made Yesterday I do a plasmid extraction.

these clones (LS GS CAD) had sent to sequencing problem with ps

digestion

I digest with PacI the plasmid that contain LS and GS

component	volume
H2O	5μl
buffer	3μl
PacI	1μl
Plasmid	20μl

Purification

Electrophoresis to isolate the insert and purification with a kit = I failed

culture

As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B

Modeling

by Arnaud, Eugène, Juliette and Pierre

Meeting about modeling with advisor Claire to talk about the achievement of our model

• Diffusion of O₂ in the gel : maths is OK but biologist need more drawings.
• Bacterial growth on the surface : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab.
• Translecture system : maybe we have to give up this part of our work because of the new project.
• Diffusion of the limonene : Eugène has focused on the concentration of limonene in the atmosphere.