Protocol 1: Produce LB Broth

Materials and Equipment

LB broth mix, sterile water, weighing scales, weighing boat, conical flask, measuring cylinder.

Time

Prep: 5 minutes
Run: 2 hours

Procedure

1. Weigh out 2g of LB broth mix in a weighing boat and add into a 250ml conical flask.
2. Measure 100ml sterile water into a measuring cylinder and dispense into the same conical flask.
3. Swirl.
4. Stopper the flask with cotton wool and foil.
5. Label on autoclave tape. Format 'iGEM, Room Number'.
6. Move the flask(s) to the autoclave to be sterilised.