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From 2014.igem.org

Contents 1 Lab Work 1.1 B-Construction of the fusion protein 1.2 D-Lemon scent 1.2.1 Limone synthase 1.2.2 PPS5 1.2.3 PPS3 and PPS4

Lab Work

B-Construction of the fusion protein

'by Hoang Vu'

We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent prick out.

D-Lemon scent

by melanie

Limone synthase

We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from 26 AugustWe do PCR of all the clones:

We use the Protocol - PCR for bacterial culture and we use the same PCR condition than on the August 8 but with the appropriate Primer and Biobrick.

PPS5

We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) So we do a PCR in the same condition than August 20

PPS3 and PPS4

We need to work on this plasmid tomorrow so we do some culture from the stock