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Team:Oxford/modelling biosensor
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System Characterisation
Predicting the mCherry fluorescence
To model the first double repression, we took the fact that we won’t need to know the amount of tetR in the system and used the assumption that ATC is effectively activating the expression of dcmR, albeit parameterised by different constants. This assumption should be justified by the fact that we will be able to precisely control the addition of ATC and we will be able to measure the fluorescence of the mCherry.
We modelled this first step using both deterministic and stochastic models.
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To model the first double repression, we took the fact that we won’t need to know the amount of tetR in the system and used the assumption that ATC is effectively activating the expression of dcmR, albeit parameterised by different constants. This assumption should be justified by the fact that we will be able to precisely control the addition of ATC and we will be able to measure the fluorescence of the mCherry.
We modelled this first step using both deterministic and stochastic models.
Oliver says:
Spent the morning checking over my repressor/activator network responses in preparation to present the data to the biochemistry department. Then had the weekly department meeting. In the afternoon we sorted out access to most areas of the Engineering department, then Skyped with the University of Sheffield about potential collaboration ideas. There is quite a lot of cross over in the human practices area and it was also suggested that we may be able to stochastically model their constitutively expressing bacteria. Finally, edited Matlab graphs for the presentation.
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