Team:Evry/Notebook/Sensing/07-29-2014

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Survivability in molecules which are going to be sensed:
Ranges of concentrations of molecules that we want to sense were made as described in following tables:
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1. Serial dilutions of compound were made

  • Lead
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  • Cadmium
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  • Nitrite
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    2. 300µL of E.coli BL21 was added in each eppendorf tube.
    We had 2 control tubes:
    *A negative control: 2,7mL of MB medium + 300µL of compound
    *A positive control: 2,7mL of MB medium + 300µL of E.coli BL21

    3. Incubation of tubes at 30°C overnight, 200rpm

    Jul 29

    • Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Sensing/07-29-2014"