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Team:BYU Provo/Notebook/Biofilm/septoct
From 2014.igem.org
BYU 2014 Notebook |
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2 September 2014
Alpha Amylase: Based off the sequencing results from the latest round of colony PCR of alpha amylase, it looks like colony 3 contained a mutated PstI restriction site. From the streak plate and overnight was started. (JB)
3 September 2014
Alpha Amylase: Performed a plasmid prep of the colony 3 mutant following the SpinSmart High-copy plasmid purification. I took the prep and measured the plasmid concentration by nano drop and it only read around 20 ng/uL. This is definitely too low to get any sequencing from. I started a new overnight from the colony 3 plate streak. (JB)
Aii: I obtained colonies on all of the plates that I used to transform the Aii and TolB into E. coli, which I hope is a good sign. My next step in the process is to do colony, which I finished today. I used the plasmid specific primers.(CZ)
4 September 2014
Alpha Amylase: Performed a plasmid prep of the colony 3 mutant again. This time I got a much larger pellet of cells from the overnight. The concentration was around 60 ng/uL however. Not sure why I have been getting such low plasmid concentrations lately. (JB)
5 September 2014
Aii: Today I am running my PCR products from Wednesday out on a standard agarose gel. (CZ)
