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Team:BYU Provo/Notebook/Biofilm/septoct
From 2014.igem.org
BYU 2014 Notebook |
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2 September 2014
Alpha Amylase: Based off the sequencing results from the latest round of colony PCR of alpha amylase, it looks like colony 3 contained a mutated PstI restriction site. From the streak plate and overnight was started. (JB)
3 September 2014
Alpha Amylase: Performed a plasmid prep of the colony 3 mutant following the SpinSmart High-copy plasmid purification. I took the prep and measured the plasmid concentration by nano drop and it only read around 20 ng/uL. This is definitely too low to get any sequencing from. I started a new overnight from the colony 3 plate streak. (JB)
4 September 2014
Alpha Amylase: Performed a plasmid prep of the colony 3 mutant again. This time I got a much larger pellet of cells from the overnight. The concentration was around . I prepped the plasmid for sequencing using the forward and reverse primer and the new internal primer. (JB)
