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From 2014.igem.org

Contents 1 LabWork 1.1 B- Construction of the fusion protein 1.2 D- Lemon scent 1.2.1 PCR LS 1.3 =Ligation

LabWork

B- Construction of the fusion protein

We made a classic exttraction of plasmids from the liquid cultures.

Check by electrophoresis if it worked and send it for sequencing.

D- Lemon scent

by Mélanie

PCR LS

Electrophoresis of the PCR made Yesterday

[photo]

The PCR have success so I use the PCR clean up kit to purify it

[photo] Electrophoresis after purification

=Ligation

We already have some pPSI digested and dephosphorelated

So I do a ligation: