Team:Evry/Notebook/Sensing/PCBs/week5
From 2014.igem.org
Week 5
Sensor construction bphR1/bphR2
'''bphR1 promoter:'''
Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
Incubation at 37°C overnight
Plate of 1 colony on LB-agar-Cam (25 ml Lb-Agar + 25µL Cam)
Incubation at 37°C overnight and glycerol stock
Sensor construction bphR1/bphR2
'''bphR1 promoter:'''Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
Incubation at 37°C overnight
Plate of 1 colony on LB-agar-Cam (25 ml Lb-Agar + 25µL Cam)
Incubation at 37°C overnight and glycerol stock