To enhance the effects of decontamination in different fields, we use Alkaline Cellulase as additive in detergent. We guide the gene of Alkaline Cellulase into the E. Coli. Triggered by P43 promoter, the targeting enzymes can be largely produced with the help of Kil, which is one of the highlight of our project. MdfA , a proton pump across the cell membrane, is added to move the proton inside the membrane, and Na+/K+ are released at the same time, thus allowing E.coli exist in PH 10. Another notable point is that we utilized the target gene to a ccdAB suicide system, we can use it to remove the biological machine whenever we need to stop, which is designed for biological safety.

The Kil protein element

Kil protein can promote the secretion of periplasmic protein, which is not limited by the molecular weight of protein. When the Kil gene is expressed, bacteria can produce more proteins than that in periplasmic cavity, which can induce the host bacterium to synthesis even more proteins. Besides, in Escherichia Coli, natural plasmid contains the genes of colicin and related substance, which can increase the permeability of outer membrane. Thus the colicin can be sent to the outside to kill other bacterias.

The ccdAB Suicide System

CcdAB system, which consists of ccdA and ccdB, is a toxin-antitoxin system. It codes CcdB, a kind of toxin protein. CcdB can interfere DNA synthesis to kill the host cell. The special Escherichia Coli is created for industrial use, so the suicide system can be controlled freely. In normal times, the channel is closed by a repressor, there would be no CcdB. While adding IPTG, the repressor’s structure changes and the channel is opened, the CcdB comes into being, and starts to kill host cell.

In papermaking industry

Alkaline Cellulase is widely used in papermaking. One of the important fibrous materials is daily waste papers. Alkaline Cellulase is needed in void treatment. Compared with chemical method, enzymatic process has many advantages, for example, the drainage properties of pulp is improved a lot, and the whiteness of paper is significantly improved.

In textile industry

Through enzymatic hydrolysis, cellulase can open nodules in cloth to make cloth more fluffy and comfortable. The surface can be made more smooth and colorful. Experience tells that alkaline cellulase is environment friendly, since it could reduce the sewage disposal and improve the washing efficiency.