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Project Description

Our project is based on genetic engineering to construct a recombinant plasmid and transform it into a host cell E.coli, given the comprehensive toxicity test ability and the function of the particular toxin detection. Use of microfluidic chips will accomplish a rapid detection of samples. Eventually, we will form a complete set of water quality toxin rapid detection system.

The system is compose of a sampling device chip, lyophilized power of recombinant strains and testing instruments. We use chips with recombinant bacteria to get the water samples, then put chips into the instrument. When the instrument works, we will acquire an automatic detection of physical and chemical characteristics of the samples after a period of time. And a test report is additional to show the final result.

The advantages of this system may be: Firstly, the recombinant host has strong resistance to toxin, providing a luminescent gene LuxAB in vivo and constructing a more stable platform for the expression of many biosensors. Secondly, microfluidic chips’ usage enhance the level of trace detection. Thirdly, inserted biosensors will completely accomplish the determination of one or several toxins, which can be taken as credible references to the comprehensive toxicity assessments. Multiple collocation of variable Biosensors also reflects the modular thought.

References

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.