Team:UT-Dallas/Notebook/8-05

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Test digest for reporter vectors August 5 %http://i.imgur.com/s2bH0st.jpg

Tuesday, August 05, 2014

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We started using Kristina home stock cell now instead of our own cell and the commercial cells.

We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.

Kristina brought lots of food: cookies, muffins, etc.

Today's tasks:

  • Transform.
  • List out mis-match of gRNA&reporter vectors.
  • Inoculate reporter vector from glycerol stock.



Preparing cells for plasmid purification.


Description of image from today