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From 2014.igem.org

Contents 1 Tuesday 8th July 1.1 Lab work 1.1.1 1 - Rehydration of the following biobricks: 1.1.2 2 - Transformation in DH5α of the following biobricks: 1.1.3 3 - Plasmid DNA preparation:

Tuesday 8th July

Lab work

1 - Rehydration of the following biobricks:

Sean

• BBa_B0015(CmR)
• BBa_J23119(CmR)
• BBa_K731020(CmR)
• BBa_K206000(CmR)
• BBa_J04500(CmR)
• BBa_J23100(AmpR)
• BBa_J23106(AmpR)
• BBa_J23114(AmpR)

Protocol:

1. Pierce foil cover of well containing biobrick of interest.
2. Add 10 μl of water in well.
3. Transfer contents of well into a 1.5 ml microcentrifuge tube.

2 - Transformation in DH5α of the following biobricks:

Mathieu & Maher

• BBa_B0015(CmR)
• BBa_J23119(CmR)
• BBa_K731020(CmR)
• BBa_K206000(CmR)
• BBa_J04500(CmR)
• BBa_J23100(AmpR)
• BBa_J23106(AmpR)
• BBa_J23114(AmpR)
• Control + : pUC18 (20ng)
• Control -

Protocol

NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.

3 - Plasmid DNA preparation:

Romain & Sean

Plasmids used:

• pJBEI-6409

E. coli strain used:

• XL1-Blue

Protocol

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