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From 2014.igem.org

Contents 1 Wednesday 16th July 1.1 Lab work 1.1.1 Transformation of competent E.coli cells 1.1.2 2 - Plasmid DNA Purification 1.2 Reunion

Wednesday 16th July

Lab work

Transformation of competent E.coli cells

by Fabio, Maher and Romain

We transformed in DH5α the following Biobrick:

• BBa_J23119

Samples:

1. Concentrated
2. Undiluted
3. Diluted 10^-1
4. Control

Results:

1. Concentrated: 99 colonies
2. Undiluted: 5 colonies
3. Diluted: 0
4. Control: 0

Conclusion:

Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.

Protocol

2 - Plasmid DNA Purification

by Mathieu

• BBa_J23100 (x2)
• BBa_J23106 (x2)
• BBa_J23114 (x2)

from Bacterial Culture made the 15th July

Protocol

Reunion

Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)

Reunion: Lab meeting to discuss the project. (afternoon)

Meeting with Evry's Team. (night) Arnaud represents us

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