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Team:Warwick/Safety
From 2014.igem.org
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RNA dependent RNA polymerase (RdRp) Background: RdRp is an enzyme which catalyzes the replication of RNA from an RNA template, an essential protein for all RNA viruses. RdRp in Hepatitis C virus is also referred to as the NS5B protein, with a total weight of 65 kDa. hete expression of NS5B has been achieved in insect and bacterial hosts, performing RNA-dependent RNA synthesis (Behrens et al., 1996; Lohmann et al., 1997). Hydrophobic C-terminal 21 amino acid residues cause insertion into the membrane, essential for HCV RNA replication (Moradpour et al., 2004). Amino acid substitution experiments of the 21 amino acid insertion sequence indicates functions beyond a membrane anchor (Moradpour et al., 2004). | ||
