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From 2014.igem.org

Contents 1 Thursday 31st July 1.1 Lab work 1.1.1 C - Salicylate Inducible Suppressing System 1.1.1.1 Transformation of competent E.coli cells 1.1.2 D - Lemon scent 1.2 Human Pratices

Thursday 31st July

Lab work

C - Salicylate Inducible Suppressing System

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α the Ligation's result made yesterday (BBa_J61051 and BBa_K228001).

Samples:

1. Sample 2 μl
2. Sample 1 μl
3. DH5α Control

Results on 1st August morning:

1. Success, grown very well (+- 100 colonies)
2. Success, grown well (+- 50 colonies)
3. Success, nothing has grown

Protocol

D - Lemon scent

by Romain

2 Precultures of DY330 with pJBEI-6409 in 2ml LB and Cm.

Human Pratices

Reunion, Handled topics:

• Organisation of the Meeting with french teams
• Great discussion about Human Practices
• Discussion about the logo

Members present:

• Instructors and advisors: Alice and Solenne.
• Students: Arnaud, Fabio, Romain, Sean and Terry.

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