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Team:NTNU Trondheim/Notebook
From 2014.igem.org
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Team:Cornell/notebook
From 2013.igem.org
Notebook
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Wet Lab
Dry Lab
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Week 23
(02/06 - 08/06)
June 3rd
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testtesttesttest test
- Prepared SOC and yB solutions for future lab work.
- Sterilized material and solutions needed for future lab work.
June 4th
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Made LB plates with ampicillin and ampicillin + kanamycin.
June 5th
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{{{tech}}}
Inoculated E. coli DH5α in SOC medium overnight.
June 6th
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OD of culture was 0.3160 after 100 minutes.
Made competent E. coli DH5α cells.
Week 24
(09/06 - 15/06)
June 10th
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Transformation was done by heat-shock. A plasmid containing ampicillin resistance was used, and the transformed cells were incubated overnight on LB plates with ampicillin. Plates showed a bacterial blanket the next day; the cells were apparently super competent.
Test of transformation efficiency of competent E. coli DH5α from June 6th.
