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From 2014.igem.org

Contents 1 Monday 4th August 1.1 Lab Work 1.1.1 A - The frame coli Odor free 1.1.1.1 Striate on Dishes 1.1.2 C - Salicylate Inducible Suppressing System 1.1.2.1 Plasmid DNA Purification 1.1.2.2 Digestion to check 1.1.2.3 Electrophoresis 1.1.3 D - Lemon scent 1.1.3.1 Gel electrophoresis of BBa_K762100 1.1.3.2 Electroporation

Monday 4th August

Lab Work

A - The frame coli Odor free

Striate on Dishes

by Juliette & Romain

We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes 1st August

C - Salicylate Inducible Suppressing System

Plasmid DNA Purification

by Eugene and Fabio

• Ligation of BBa_J61051 and BBa_K228001 made the 30th July

From Liquid Culture made the 1st August

Digestion to check

In progress

Electrophoresis

In progress

D - Lemon scent

Gel electrophoresis of BBa_K762100

by Sean

Samples used were prepared on the 30th July.

Results

From left to right: 5 µl of BBa_K762100 + "old" Phusion enzyme, 5 µl of BBa_K762100 + "new" Phusion enzyme

Electroporation

by Terry

Strain used: DY330 containing plasmid pJBEI6409.

We had to switch the Limonen Synthase cassette with the Apramycin resistance.

The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.

Members there:

• Instructors and advisors: Alice, Solenne and Sylvie.
• Students: Eugene, Fabio, Hoang Vu, Juliette, Pierre, Romain, Sean and Terry.

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