Team:ULB-Brussels/Project/Methods

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- Université Libre de Bruxelles -



Material and Methods


Some technical material used during our genetical experiments will be emphasized hereabouts.

Different kinds of experimental methods have been applied in our Lab too.

Abc .

First, birth and growing of bacteria populations (Centrifugation, Dilution).

Secondly, insertion of the biobricks and plasmids chosen with E. Coli (Electroporation method, PCR Amplification, Electrophoresis), bacteria selection (in function of the quantities od oligopeptids or phoA+prolin), inclusion of a toxin-antitoxin (TA: ccdB-ccdA) system in E. Coli.

Just after this step, addiction of fluorescent proteins (GFP, RFP) and determination of the quantities and properties of our bacteria (including Emission Spectroscopy) + analize of their genetical sequences.

Finally, conservation of bacteria populations that product the desired molecules in good quantities, at cold temperature in containers.