Team:Paris Saclay/Notebook/July/29

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Contents

Tuesday 29th July

Lab Work

A - The frame

PCR

by Romain

StrainS used: E. coli MG1655Z1 and E. coli MG1655.

Protocol:

  • 218µl of H20 MilliQ
  • 80µl GoTaq buffer 5X
  • 16µl oligonucleotide FTR-Apra-F (10mM)
  • 16µl oligonucleotide FTR-Apra-R (10mM)
  • 12µl DMSO 10mM
  • 32µl MgCl2 (25mM)
  • 8µl dNTP (10mM)
  • 2µl of GoTaq enzyme
  • 2µl of different bacterial cultures in each tubes:
  1. Tube 1: MG1655Z1 10I
  2. Tube 2: MG1655 100II
  3. Tube 3: MG1655 50I
  4. Tube 4: MG1655 100I
  5. Tube 5: MG1655Z1 10II
  6. Tube 6: MG1655 50II
  7. Tube 7: MG1655Z1 10I positive control with plasmid

Members there:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Romain, Sean and Terry.

Back to the calendar

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