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Team:Paris Saclay/Notebook/July/24
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Contents |
Thursday 24th July
Lab work
B - Construction of the fusion protein (color)
Plasmid DNA Extraction
by Terry
Biobricks used:
- 1. BBa_K1033927 with pSB1C3 (pink chromoprotein)
- 2. BBa_K1033910 with pSB1C3 (yellow chromoprotein)
- 3. BBa_K1033921 with pSB1C3 (red chromoprotein)
- 4. BBa_K1033922 with pSB1C3 (red chromoprotein)
- 5. BBa_K1033902 with pSB1C3 (blue chromoprotein)
- 6. BBa_K731201 with pSB1C3 (Arabinose inductible araC-pBAD promoter)
- 8. BBa_K1033925 with pSB1C3 (pink chromoprotein)
- 9. BBa_K1033905 with pSB1C3 (purple chromoprotein)
- 10. BBa_K1033913 with pSB1C3 (orange chromoprotein)
- 11. BBa_K1033929 with pSB1C3 (blue chromoprotein)
C - Lemon scent
Plasmid DNA Extraction
by Terry
Biobricks used:
- 7. BBa_K762100 with pSB1C3 (Limonen synthase1 with RBS)
Gel electrophoresis of p cola
by Sean
p cola used: sample left overnight yesterday resuspended in 30μl of H2O milliQ.
Results
Results of the PCR Targeting
by Romain
PCR targeting made the July 22nd.
Results:
- Nothing has grown on the control dishes.
- Failure, nothing has grown on the clone I dishes.
- Failure, other bacteria have grown on the clone II dishes.
We have to make another PCR.
E - Salicylate Inducible Suppressing System
Plasmid DNA Extraction
by Terry
Biobricks used:
- 12 & 13. BBa_J45017 with pSB1C3 with pSB1AK3 (pchBA synthase (converts chorismate to salicylate))
Transformation of competent E.coli cells
by Fabio
We transformed in DH5α both BioBricks rehydrated yesterday (BBa_J61051 and BBa_K228001).
Samples:
- BBa_J61051 Concentrated
- BBa_J61051 Undiluted
- BBa_K228001 Concentrated
- BBa_K228001 Undiluted
- DH5α Control
Members present:
- Instructors and advisors: Alice and Solenne.
- Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.
