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Team:Paris Saclay/Notebook/June/30
From 2014.igem.org
Contents |
Monday 30th June
Lab work
1 - Culture or the supercompetents:
by Mathias
- MG1655
- MG1655Z1
1ml of an overnight culture in 100ml of LB.
2 - Réhydration of selected biobricks:
by Juliette
- BBQ_I9026
- BBQ_K592025
- BBQ_K786002
- BBQ_K786003
- BBQ_K778002
- BBQ_K592011 {2 times}
- BBQ_K592009
- BBQ_K1033910 {idem}
- BBQ_J45014
- BBQ_K517003
3 - Transformations :
by Maher & Mathias
We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10-1 and 10-2.
4 - Preparations :
- Aliquots of antibiotics :
by Marie & Mathieu
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)
- Petri dishes
by Romain
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.
- Range of gelose with different concentration of agar
by Arnaud
Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.
Members present:
- Instructors : Solenne.
- Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
