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Team:Paris Saclay/Notebook/July/8
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Contents |
Tuesday 8th July
Lab work
1 - Rehydration of the following biobricks:
Sean
- BBa B0015(CmR)
- BBa J23119(CmR)
- BBa K731020(CmR)
- BBa K206000(CmR)
- BBa J04500(CmR)
- BBa J23100(AmpR)
- BBa J23106(AmpR)
- BBa J23114(AmpR)
Protocol:
- Pierce foil cover of well containing biobrick of interest.
- Add 10 μL of water in well.
- Transfer contents of well into a 1.5 mL microcentrifuge tube.
2 - Transformation in DH5α of the following biobricks:
Mathieu & Maher
- BBa B0015(CmR)
- BBa J23119(CmR)
- BBa K731020(CmR)
- BBa K206000(CmR)
- BBa J04500(CmR)
- BBa J23100(AmpR)
- BBa J23106(AmpR)
- BBa J23114(AmpR
- Control + : pUC18 (20ng)
- Control -
NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.
3 - Plasmid DNA preparation:
Romain & Sean
Plasmids used:
- pJBEI-6409
E. coli strain used:
- XL1-Blue
