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From 2014.igem.org

Contents 1 Monday 30th June 1.1 Lab work 1.1.1 1 - Culture or the supercompetents: 1.1.2 2 - Réhydration of selected biobricks: 1.1.3 3 - Transformations : 1.1.4 4 - Preparations :

Monday 30th June

Lab work

1 - Culture or the supercompetents:

by Mathias

• MG1655
• MG1655Z1

1mL of an overnight culture in 100mL of LB.

2 - Réhydration of selected biobricks:

by Juliette

• BBQ_I9026
• BBQ_K592025
• BBQ_K786002
• BBQ_K786003
• BBQ_K778002
• BBQ_K592011 {2 times}
• BBQ_K592009
• BBQ_K1033910 {idem}
• BBQ_J45014
• BBQ_K517003

3 - Transformations :

by Maher & Mathias

We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10^-1 and 10^-2.

4 - Preparations :

• Aliquots of antibiotics :

by Marie & Mathieu

Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)

• Petri dishes

by Romain

Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.

• Range of gelose with different concentration of agar

by Arnaud

Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.

People there:

• Instructors : Solenne.
• Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.

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