Team:Freiburg/Content/Notebook
From 2014.igem.org
ID | Template | Primer 1 | Primer 2 | Size[kbp] | |
PCR 1 | pKM292 | o14zl_039 | o14zl_040 | 1,3 | p14zl_010_pLXV_Gal4BD_LOV_puro |
PCR 2 | p14zl_003 | o14zl_041 | o14zl_042 | 0,66 | p14zl_010_pLXV_Gal4BD_LOV_puro |
PCR 3 | pQXIN SLC7A1 | o14rz_016 | o14rz_017 | 1,87 | p14rz_003_pcDNA_SLC71A_GFP |
PCR 4 | pQXIN SLC7A1 | o14rz_018 | o14rz_019 | 1,87 | p14rz_005_pcDNA_SLC7A1_mCherry |
PCR 5 | pKM078 | o14rz_020 | o14rz_021 | 0,78 | p14rz_005_pcDNA_SLC7A1_mCherry |
PCR 6 | p14rz_002 | o14rz_022 | o14rz_023 | 1,93 | p14rz_004_pcDNA_SLC7A1_HA |
PCR 7 | pKM084 | o14_ls_013 | o14_ls_014 | 3,9 | p14ls_002_Gal4_mCherry |
PCR 8 | pKM078 | o14_ls_015 | o14_ls_016 | 0,78 | p14ls_002_Gal4_mCherry |
PCR 9 | pQXIN SLC7A1 | o14_ls_017 | o14_ls_018 | 1,93 | p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry |
PCR 10 | pBK001 | o14_ls_019 | o14_ls_020 | 0,87 | p14ls_003_Gal4_SLC7A1_HA_2a_Tag_mCherry |
PCR 11 | pKM022 | o14_ls_005 | o14_ls_006 | 2,8 | p14ls_001_COP1-VP16_TetR_UVR8 |
PCR 12 | Digest 7 | o14_ls_008 | o14_ls_009 | 1,4 | p14ls_001_COP1-VP16_TetR_UVR8 |
PCR 13 | UVR8 (Bba_1150029) | o14_ls_010 | o14_ls_011 | 1,2 | p14ls_001_COP1-VP16_TetR_UVR8 |
PCR 14 | psi_pack_synthese | o14vv_031 | o14vv_032 | 0,64 | pMIG_Standardisierung (Synthese rein) |
PCR 15 | p14vv_003_backbone | o14vv_033 | o14vv_034 | 3,4 | pMIG_Standardisierung (XbaI raus) |
PCR 16 | COP1_VP16(Patrick) | o14_ls_007 | o14_ls_012 | 1,5 | p14ls_001_COP1-VP16_TetR_UVR8 |
PCR 17 | pBK001 | o14_rz_028 | o14_rz_029 | 0,84 | p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry |
PCR 18 | pQXIN SLC7A1 | o14_rz_026 | o14_rz_027 | 1,9 | p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry |
PCR 19 | pKM297 | o14zl_035 | o14zl_036 | 1,5 | p14zl_011_pLXV_ePDZ_VP16_mCherry |
PCR 20 | pKM078 | o14zl_037 | o14zl_043 | 0,8 | p14zl_011_pLXV_ePDZ_VP16_mCherry |
PCR 21 | pCDNA | o14rz_024 | o14rz_025 | 5,4 | p14rz_004_pcDNA_SLC7A1_HA_2 |
PCR 22 | pQXIN SLC7A1 | o14rz_030 | o14rz_031 | 1,9 | p14rz_004_pcDNA_SLC7A1_HA_2 |
PCR 23 | pQXIN SLC7A1 | o14rz_032 | o14rz_033 | 1,9 | p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry |
PCR 24 | pBK001 | o14rz_034 | o14rz_035 | 0,85 | p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry |
PCR 25 | p14zl_003 | 014zl_010 | 014zl_011 | 5,4 | p14zl_004_pMIG_Phyb_VP16_puro_TetR_PIF6 |
PCR 26 | pKM006 | o14vv_037 | o14vv_038 | 1,6 | p14vv_006_pMIG_CMV_SEAP |
PCR 27 | pKM006 | o14vv_037 | o14vv_039 | 1,6 | p14vv_007_pMIG_SEAP |
PCR 28 | pLXV | o14zl_044_pLXV_rv_neu | o14zl_030 | 6,3 | p14zl_008_pLXV_TetO13_CMVmin_SLC7A1 |
PCR 29 | p14rz_006 | o14zl_048 | o14zl_049 | 2,7 | p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry |
PCR 30 | p14vv_003_backbone | o14zl_050 | o14zl_051 | 4,9 | p14zl_006_pMIG-TetO13_CMVmin_SLC7A1_HA_p2a_mCherry |
PCR 31 | lenti-CRISPR | o14zl_020 | o14zl_021 | 0,7 | p14zl_007_pLXV_LOV_ePDZ_puro |
PCR 32 | pKM297 | o14zl_022 | o14zl_023 | 1 | p14zl_007_pLXV_LOV_ePDZ_puro |
PCR 33 | pKM292 | o14zl_024 | o14zl_025 | 1,4 | p14zl_007_pLXV_LOV_ePDZ_puro |
PCR 34 | pKM022 | o14zl_026 | o14zl_027 | 0,6 | p14zl_007_pLXV_LOV_ePDZ_puro |
PCR 35 | p14rz_006 | o14rz_033 | o14rz_036 | 2 | p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry |
PCR 36 | pGEMHE-XfA4-mCherry | o14rz_035 | o14rz_037 | 0,8 | p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry |
PCR 37 | p14rz_006 | o14rz_026 | o14rz_036 | 2 | p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry |
PCR 38 | pBK001 | o14rz_029 | o14rz_037 | 0,8 | p14rz_007_pcDNA_SLC7A1_HA_linker_mCherry |
PCR 39 | pKM084 | o14rz_040 | o14rz_041 | 3,9 | p14rz_009_Gal4_SLC7A1-HA |
PCR 40 | p14rz_002 | o14rz_039 | o14rz_038 | 2 | p14rz_009_Gal4_SLC7A1-HA |
PCR 41 | pMIG | o14rz_042 | o14rz_043 | 1,4 | p14rz_012_pcDNA_SLC7A1_IRES_GFP |
PCR 42 | pQXIN SLC7A1 | o14rz_044 | o14rz_045 | 2 | p14rz_012_pcDNA_SLC7A1_IRES_GFP |
PCR 43 | mTAG_BFP2 | o14vv_044 | o14vv_045 | 0,8 | p14vv_008_pMIG_mTAG_BFP2 |
PCR 44 | mKO | o14vv_042 | o14vv_043 | 0,8 | p14vv_009_pMIG_mKO |
PCR 45 | mKate | o14vv_046 | o14vv_047 | 0,8 | p14vv_010_pMIG_mKate |
PCR 46 | pQXIN SLC7A1 | o14vv_050 | o14vv_051 | 2 | p14vv_012_pMIG_SLC7A1_IRES_GFP |
PCR 47 | p14zl_003 | o14vv_054 | o14vv_055 | 0,7 | p14vv_013_pMIG_eGFP_IRES_puro |
PCR 48 | p14zl_003 | o14vv_052 | o14vv_053 | 0,7 | p14vv_013_pMIG_eGFP_IRES_puro |
PCR 49 | pMIG | o14vv_056 | o14vv_057 | 0,7 | p14vv_013_pMIG_eGFP_IRES_puro |
PCR 50 | pMIG | o14vv_056 | o14vv_059 | 0,8 | p14vv_014_pMIG_eGFP_p2a_puro |
PCR 51 | p14zl_003 | o14vv_055 | o14vv_058 | 0,8 | p14vv_014_pMIG_eGFP_p2a_puro |
PCR 52 | p14zl_003 | o14zl_052 | o14zl_053 | 2,4 | p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6 |
PCR 53 | p14zl_003 | o14zl_054 | o14zl_055 | 2,4 | p14zl_016_pMIG_PhyB-VP16_puro_TetR-PIF6 |
PCR 54 | p14ls_001 | o14zl_056 | o14zl_057 | 4 | p14zl_015_pMIG_COP1-VP16_TetR_UVR8 |
PCR 55 | p14rz_008_Gal4_SLC7A1 | p14zl_061 | p14zl_062 | 0,27 | p14zl_018_pLXV_Gal4_GFP_WPRE |
PCR 56 | pEGFP-C1 | p14zl_063 | p14zl_064 | 0,83 | p14zl_018_pLXV_Gal4_GFP_WPRE |
PCR 57 | pMIG-venus | p14zl_058 | p14zl_059 | 1,37 | p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE |
PCR 58 | p14rz_008_Gal4_SLC7A1 | p14zl_061 | p14zl_065 | 2,13 | p14zl_019_pLXV_Gal4_SLC7A1_venus_WPRE |
PCR 59 | pMIG-dsRED | p14zl_069 | p14zl_068 | 1,34 | p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED |
PCR 60 | pKM292 | p14zl_066 | p14zl_067 | 1,4 | p14zl_020_pLXV_SV40_Gal4BD_LOV_IRES_dsRED |
PCR 61 | pMIG-Venus | p14zl_059 | p14zl_076 | 0,61 | p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE |
PCR 62 | pGEMHE-XfA4-mKate | o14zl_074 | p14zl_075 | 0,78 | p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE |
PCR 63 | p14rz_008 | p14zl_061 | p14zl_065 | 2,1 | p14zl_021_pLXV_Gal4_SLC7A1_mKate_WPRE |
PCR 64 | pMIG-CFP | p14zl_059 | p14zl_058 | 1,37 | p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE |
PCR 65 | pKM297 | o14zl_035 | p14zl_060 | 1,5 | p14zl_017_pLXV_SV40_ePDZb-VP16_IRES_CFP_WPRE |
PCR 66 | p14rz_008_Gal4_SLC7A1 | p14zl_070 | p14zl_065 | 2,1 | p14zl_022_pMIG_Gal4_SLC7A1_venus |
PCR 67 | pMIG-Venus | p14zl_059 | p14zl_073 | 1,37 | p14zl_022_pMIG_Gal4_SLC7A1_venus |
PCR 68 | p14rz_008_Gal4_SLC7A1 | p14zl_070 | p14zl_062 | 0,26 | p14zl_023_pMIG_Gal4_GFP |
PCR 69 | pEGFP-C1 | p14zl_072 | p14zl_071 | 0,83 | p14zl_023_pMIG_Gal4_GFP |