5/19/14

• Calibrated Nanodrop.
• Nanodropped all of our samples. Very low concentration of DNA.
• Ordered primers to PCR our g-blocks.

5/22/14

• Struck out plates with pACYC184 with spectinomycin to grow it.
• Digested pBR322 with HindIII.
• Double digested pBR322 with HindIII/EcoRI.

5/23/14

• Ran gel of digests from 5/22/14. Everything as it should be.
• Purified the digested plasmids.
• Nanodropped plasmids; results:

  Single digest I: 52.7ng/μL

  Single digest II: 64.5ng/μL

  Double digest I: 26.3ng/μL

  Double digest II: 41.9ng/μL

• Phosphatase treated the four digested plasmids.

5/27/14

• Purified phosphatase treated plasmids from 5/23/14.
• Nanodropped the purified plasmids; results:

  Single digest I: 43.4ng/μL

  Single digest II: 53.6ng/μL

  Double digest I: 43.7ng/μL

  Double digest II: 16.5ng/μL

5/28/14