Team:Toulouse/Result/achievement
From 2014.igem.org
Achievement
Bronze, Silver or Gold Medal? That is the question!
Results > Achievement
Let's sum up what we did during this summer!
First step...
After a summer of hard work, our team is proud of the achieved results. The first step of our project was to have a proof of concept of the three functions requiered to fight against canker. The three modules tested were Chemotaxis, Binding and Fungicides. The verifications of our genetic constructions were successful (PCR, Migration on an agarose gel and Sequencing). We could then proceed to specific test of the modules: capillary test, chitin beads test or fungicide tests.
Second step...
Once these results obtained, we decided to move on to the next step: in order to get closer to our final objective, the three genetic modules have to be assembled and to be tested in model plants. In the short time left that we had, these tests were only performed with the antifungal module, but the results were very encouraging (See 3. In planta tests with SubtiTree in the Fungicides module).
Third step...
So encouraging that we moved on to the third step of our project and think about the real injection of SubtiTree in diseased trees. We then considered the spreading problems and proposed different strategies to avoid this major issue. Modeling enables us to predict the growth and the lifespan of SubtiTree in Plane Trees.
And what comes next?
This project could be continued thanks to a thesis as the problem of canker killing plane trees is a major concern. Moreover, the support of the Ministry and VNF could help us to continue and achieve our goal: save the plane trees and preserve the beauty of the Canal du Midi.
However, SubtiTree dissemination must be controlled, fungicide production regulated, and tests of the whole system performed. We also need to ask that the fungicides chosen could be used according to the legislation. In a nutshell, we still have a lot of things to do even if we did our best during all the summer!
iGEM Community
Well aware that we are part of the iGEM Community, our team also worked for this community by improving the iGEMer's Bible: the Registry of Standard Biological Parts.
We improved the EcAMP BioBrick by adding a promotor, a RBS, but mainly by the addition of a STOP codon (BBa_K1364019).
As Bacillus subtilis appears to be for us an ideal chassis, we also developed new genetic tools to facilitate its utilization. Thanks to numerous interactions with other iGEM teams, we didn't see the time flying by during this amazing summer !
![](https://static.igem.org/mediawiki/parts/e/e2/Ach.jpg)
It is better to have "well-made rather than a well-filled head"... (Michel de Montaigne)
We developed a deep ethical questioning, trying to understand if we had the right to act against canker. We strongly believe that neglecting the ethical aspect in a scientific project is a mistake. All the team was involved in this part and the reflexions made were very profitable to us.
Medals Fulfillment
Bronze Medal ![]() |
Silver Medal ![]() |
Gold Medal ![]() |
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![]() BBa_K1364005, BBa_K1364009, BBa_K1364013. |
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