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Team:Oxford/modellingA
From 2014.igem.org
[DCM] calibration for solution-vapour equilibration
Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a real binary solution/ideal gas, based on literature solubility data, confirming negligible [DCM] deviation:
|
|
V(DCM) added (± 5E-4) /ml |
n(DCM)(l) unadjusted (± 8E-6) /mol |
[DCM] unadjusted (± 2E-3) /M |
p(DCM) (± 4E-2) /kPa |
n(DCM)(g) (± 4E-7) /mol |
n(DCM)(l) (± 9E-6) adjusted /mol |
[DCM] adjusted (± 2E-3) /M |
Δ[DCM] /M |
[DCM] % deviation |
|
(30°C, 5mM) |
6.4E-03 |
1.0E-04 |
2.0E-02 |
6.2E-01 |
1.6E-08 |
1.0E-04 |
2.0E-02 |
3.1E-06 |
1.6E-02 |
|
(30°C, 10mM) |
3.2E-03 |
5.0E-05 |
1.0E-02 |
3.1E-01 |
7.9E-09 |
5.0E-05 |
1.0E-02 |
1.6E-06 |
1.6E-02 |
|
(30°C, 20mM) |
1.6E-03 |
2.5E-05 |
5.0E-03 |
1.6E-01 |
3.9E-09 |
2.5E-05 |
5.0E-03 |
7.9E-07 |
1.6E-02 |
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|
(37°C, 5mM) |
6.4E-03 |
1.0E-04 |
2.0E-02 |
6.2E-01 |
1.5E-08 |
1.0E-04 |
2.0E-02 |
3.1E-06 |
1.5E-02 |
|
(37°C, 10mM) |
3.2E-03 |
5.0E-05 |
1.0E-02 |
3.1E-01 |
7.7E-09 |
5.0E-05 |
1.0E-02 |
1.5E-06 |
1.5E-02 |
|
(37°C, 20mM) |
1.6E-03 |
2.5E-05 |
5.0E-03 |
1.6E-01 |
3.8E-09 |
2.5E-05 |
5.0E-03 |
7.7E-07 |
1.5E-02 |
More detailed system modelling is therefore unnecessary.
