Team:TCU Taiwan/Attributions

Revision as of 22:00, 17 October 2014 by House (Talk | contribs)

We would like to thank all the people who came to our progress presentations and gave useful feedback.

Instructors support

Lin,Guang-Huey Professor Lin teaches knowledge about microbiology for TCU iGEM team each year, and she also guides our experimental design. Besides, she provides her lab for us to working on our experiment.		Lin,Nien-Tsung Prof. Lin, a gracious teacher, is the guiders of our experiment on M13 phage display. She provides us a lot knowledge about M13 phage and DNA cloning.

Ching,Yung-Hao Prof. Ching is one of the leader teachers of our team. She is a kind teacher who always encourages us, and she also gives us much advice and direction on safety concerns.		Woon,Peng-Yeong Prof. Woon is one of the leader teachers for this year. He helps us the basic concept of iGEM and reminds us many iGEM rules or requests we nearly missed. He is a lively professor.

Experiment

Bobo, Regina, Denise, Eating

Contributions

Transformation ( Cas9 in pSB1C3 into DH5α) Extract plasmid with pSB1C3 from DH5α. Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011. Use Enzyme digestion (PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted. Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate. Ligation of RFP and pBluescript SK(-). Transformation clone RFP/pBluescript SK(-) to DH5α. Extract plasmid clone RFP/pBluescript SK(-).
Experiment Design Ted and Bobo Design gRNA for AmpR.
Biobricks Design Ted Design the gRNA for different resistance gene.

Modeling

House, Bobo, Chao-Di Chang(National Chiao Tung University)

Contributions

Infectional efficiency ratio of M13KO7. Release titer.(Releasing titer of phagemid which is E.coli infected by helper phage)
Special Thanks Modeling Advisor Chang,Chao-Di Thanks for Chang,Chao-Di strong support on our modeling.

Human Practice

Eating

Contributions

Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener. Contact with National Chiao Tung University for cooperation.

Wiki

House

Contributions

Construct the wiki page.

Coding,coding and coding the wiki page.

Study all about Synthetic Biology from wetlab to drylab.

And last but not least

Art Design Denise, Regina, Eating, House Design of poster and PPT. Design of team uniform.		Photo & Video Sharon, Eating Take photo and video. Arrange all photos.
Administeration Eating Apply for the allowances. Arrange all files.

Video support

Wang, Yiru and Chen, Jiawei

we should be obliged to them for their assistance in this matter.

Wang, Yi-ru	Chen, Jia-wei

Special Thanks

Our partner : NCTU_Formosa

We are extremely appreciative of NCTU_Formosa that helped and support us. The model1 courtesy of Chao-Di Chang.



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