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Team:Melbourne/Safety
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Safety
1. Safety Level Rating System
2. Work Environments Used to Handle Biological Materials
3. Personal Protective Equipment
4. Disposal of Biological Waste
5. Safety Training
6. Local Rules and Regulations
7. Organisms and Parts Used
8. Risks of Our Project Now
9. Risks of Our Project in the Future
If our project is successful it will allow people to use the molecules we make as a platform technology for making disulphide bonded peptides. These peptides could potentially be used for harmful purposes. We don’t currently have any design features to reduce the risk of someone using our project for harmful purposes. In the future if it were deemed necessary we could potentially try to limit the types of amino residues or groups that could be added to the arms of our peptide by native chemical ligation.
Australia uses a four-part ‘Safety Level’ rating system for laboratories in which Level 4 is used for the most dangerous organisms. Our laboratory is Level 2 (moderate risk).
Open benches and a laminar flow hood/biosafety cabinet with open front are used when handling biological materials in our laboratory.
Some materials are handled in different work environments, for example DNA gels containing EtBr are only used in designated areas and some procedures involving bacteria that need to be sterile are performed near an open flame, while most other procedures are performed at open benches.
In our laboratory lab coats, gloves and safety glasses/goggles are worn at all times and full face shields are worn when using the UV light box. Latex gloves are used for most procedures in the lab, however, nitrile gloves are worn when handling EtBr.
Biological waste (e.g. pipette tips) are disposed of in a yellow biohazard which is sealed when not in use. When full, this biohazard bin is sent to be autoclaved before it is disposed with appropriately along with the biological waste from the other laboratories in the institute.
Biological waste such as liquid cell cultures has bleach added to it and sits overnight in the fume cabinet before the liquid is poured down a special biological waste drain and the container is autoclaved.
The Melbourne iGEM team received safety training provided by The Bio21 Institute. The topics covered in this training included: details of emergency actions, the university OH&S policy and issue resolution procedures, the university's environment policy and procedures, procedures for reporting incidents/near misses, hazards and unsafe acts/work processes, introduction to Bio21 EHS staff, location of relevant EHS documentation, security, 'out of normal hours' access, equipment operating procedures, general safety.
The laboratory safety requirements of the institution can be found here: https://intranet.mdhs.unimelb.edu.au/ohse-training-requirements.
Christian Rantzau (Bio21 Health & Safety Representative) is responsible for biological safety at our institution. We briefly discussed the program with him and no concerns were raised, nor changes made to the project. He ensured that we were appropriately trained in the safety protocols and procedures of the institution.
The biosafety guidelines of The Bio21 Institute can be found at: http://www.bio21.unimelb.edu.au/bio-21-institute-intranet/environmental-health-and-safety/safety.
The regulations that govern biosafety in research laboratories in Australia may be found via the following link: http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/Content/legislation-2.
Species Name |
Risk |
Risk Group |
Disease risk |
How did you acquire it? |
How will you use it? |
E. coli, BL21(DE3) |
1 |
E. coli K12 |
Very low virulence |
Donation from neighboring lab |
This is one of our chassis |
E. coli, SHuffle T7 |
1 |
E. coli B |
Very low virulence |
Acquired from local New England |
This is one of our chassis |
Part number/name |
Natural function |
How did you acquire it? |
Synthetic peptide 1 based on a de novo amino acid sequence |
Not natural |
Designed DNA from scratch; DNA synthesised by Life Technologies |
Synthetic peptide 2 based on a de novo amino acid sequence |
Not natural |
Designed DNA from scratch; DNA synthesised by Life Technologies |
Synthetic peptide 3 based on a de novo amino acid sequence |
Not natural |
Designed DNA from scratch; DNA synthesised by Genscript |
Synthetic peptide 4 based on a de novo amino acid sequence |
Not natural |
Designed DNA from scratch; DNA synthesised by Genscript |
Synthetic peptide 5 based on a de novo amino acid sequence |
Not natural |
Designed DNA from scratch; DNA synthesised by Genscript |
TEV protease gene |
Tobacco etch |
Donation from neighboring lab |
Risk to the Safety and Health of Team Members/People Working in the Lab
E. coli has a low, but not non-existent virulence. For this reason, we wear gloves and lab coats and wash our hands when entering and leaving the labs. When analysing DNA agarose gels both the EtBr solution and the UV light box pose a risk to the health of our team members. In these specific cases we wear nitrile gloves when handling the gel and a face shield when operating the light box.
Risk to the Safety and Health of the General Public
E. coli has a low, but not non-existent virulence. For this reason, we wear gloves and lab coats and wash our hands when entering and leaving the labs. This helps to reduce the risk to the safety and health of the general public.
Risk to the Environment
When disposing of things such as liquid cell culture, we sit them overnight in the fume cabinet with some bleach in them. We pour the liquid down the shoot in the fume cabinet on the next day and autoclave the containers. We do not pour hazardous chemicals down the sink. All of these action minimise the risks to the environment.
Risks to Security Through Malicious Mis-Use
Our project does not pose much of a risk in terms of malicious mis-use by others, but to try and prevent this we have key card access into both the building and the labs so that they cannot be accessed by the general public.
Measures Taken to Reduce Risks
We employ safe lab practices (i.e. wearing gloves and lab coats), dispose of waste appropriately, use a baceterium (E. coli) with a very low virulence, and have key card access to the labs.
