For iGEM 2014, the UT Austin team has biobricked two non-canonical amino acid (ncAA) tRNA synthetases with their cognate tRNAs. There are now three ncAA tRNA synthetases biobricked, including one from last year's UT Austin team.
[http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=Austin_Texas Group Parts - 2014]
The 2014 UT Austin iGEM team felt quite strongly that we should make our ncAA reporter kit widely available as soon as possible. Unfortunately, this complex plasmid is not BioBrick legal, and after considerable consideration and e-mailing with Vinoo Selvarajah (iGEM HQ), we decided to include the relevant information here and be willing to share the kit plasmids (and cultures) with anyone who requests it until such time as an alternative solution is found.
pFRYC - Reporter plasmid with linker containing tyrosine codon.
pFRY - Reporter plasmid with linker containing amber codon.
The two plasmids must be grown in parallel cultures. pFRYC controls for variance in gene expression dependent on the plasmid in the specific growth conditions and the length of the fusion protein. pFRY tests the fidelity and efficiency of the tRNA sythetase/tRNA pair.
Additional details for using the kit and experimentals can be found on our https://2014.igem.org/Team:Austin_Texas/kit Expanded Genetic Code Measurement Kit page].
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