Team:Oxford/Parts
From 2014.igem.org
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Oxford iGEM has submitted three parts to the registry, which are described on the relevant registry pages.
Part no. 1 (BBa_K1446001) is the gene coding for DcmA with an N-terminal pdu-microcompartment tag.
Part no. 2 (BBa_K1446002) is a superfolder GFP-gene with the N-terminal pdu-microcompartment tag. We have included a ribosome binding site upstream of the coding region as well as a his-tag at the C-terminus. This part has been used for fluorescent image analysis to show that the targeting mechanism of the N-terminal microcompartment tag works as expected.
Part no. 3 (BBa_K1446003) is the dcmR gene with a tetR promoter. We have used this part to get fluorescence data for our model described here and showed that mCherry expression increased at higher ATC (anhydrous tetracycline) concentration, as expected.
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