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We are using Bacillus subtilus as a backbone to incorporate the entire
genome of Streptococcus thermophilus. Once incorporated, we will use the
cre/lox recombination system to remove the Bacillus subtilus genome so
only the Streptococcus thermophilus genome remains. This will demonstrate
the use of Bacillus subtilus as a useful chassis to boot up an artificial
genome.
As you may have guessed from the sly shift in tense from present to future, we
did not actually succeed in transferring the entire genome of Streptococcus thermophilus
into Bacillus subtilus. We have developed, and will describe, a method which
we believe will enable us to do so, and the results we have obtained for the early
steps in that method have been encouraging. We still believe it may be possible to
accomplish our original goal, and will continue to pursue it in the weeks and months
to come.
References
iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.
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You can use these subtopics to further explain your project
- Overall project summary
- Project Details
- Materials and Methods
- The Experiments
- Results
- Data analysis
- Conclusions
It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.
It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.
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